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Gene–based sequence–tagged–sites (STSs) as the basis for a human gene map

Nature Genetics volume 10, pages 415–423 (1995)Cite this article

Abstract

Using our data set of 3,143 single pass sequences from human brain cDNA libraries, we have developed a strategy in which gene–based sequence–tagged–sites (STSs), derived from 3′untranslated regions of human cDNAs, are rapidly assigned to megabase–insert yeast artificial chromosomes and somatic cell hybrids to generate regional gene mapping data. Employing this approach, we have mapped 318 cDNAs, representing 308 human genes. Ninety–two of these mapped to regions implicated in human genetic diseases, identifying them as candidate genes. Extension of this strategy has the potential to result in virtually every human gene having, at its 3′ end, its own associated STS, with each STS in turn specifying both a corresponding genomic clone and a specific regional location in the genome.

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Author information

Author notes

  1. Richard Goold

    Present address: Incyte Pharmaceuticals, Palo Alto, California, 94304, USA

  2. Rebecca Berry and T.J. Stevens: These two authors contributed equally to this work.

Authors and Affiliations

  1. Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado, 80262, USA

    Rebecca Berry, T.J. Stevens, Nicole A.R. Walter, Andrea S. Wilcox, Todd Rubano, Janet A. Hopkins & James M. Sikela

  2. Department of Molecular Biology Program, University of Colorado Health Sciences Center, Denver, Colorado, 80262, USA

    Rebecca Berry & James M. Sikela

  3. Veterans Administration Schizophrenia Center, Denver, Colorado, 80262, USA

    Janet A. Hopkins

  4. Marshfield Medical Research Foundation, Marshfield, Wisconsin, 54449–5790, USA

    James Weber

  5. Human Genome Mapping Center, Stanford University, Stanford, California, 94305, USA

    Richard Goold

  6. Department of Psychiatry, College of Physicians & Surgeons of Columbia University and The New York State Psychiatric Institute, New York, New York, 10032, USA

    Marcelo Bento Soares

Authors
  1. Rebecca Berry
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  8. Richard Goold
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  9. Marcelo Bento Soares
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  10. James M. Sikela
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Berry, R., Stevens, T., Walter, N. et al. Gene–based sequence–tagged–sites (STSs) as the basis for a human gene map. Nat Genet 10, 415–423 (1995). https://doi.org/10.1038/ng0895-415

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