Content-Length: 121829 | pFad | http://rcsb.org/structure/9EV1

RCSB PDB - 9EV1: 3DFlex refinement of the CryoEM structure of DeCLIC nanodisc with 10mM EDTA in sym-like state

9EV1 | pdb_00009ev1

3DFlex refinement of the CryoEM structure of DeCLIC nanodisc with 10mM EDTA in sym-like state

PDB DOI: https://doi.org/10.2210/pdb9EV1/pdb
EM Map EMD-19991: EMDB EMDataResource

Classification: TRANSLOCASE
Organism(s): Desulfofustis sp. PB-SRB1
Expression System: Escherichia coli
Mutation(s): No

Deposited: 2024-03-28 Released: 2025-04-09
Deposition Author(s): Fan, C., Howard, R.J., Lindahl, E.
Funding Organization(s): Swedish Research Council

Experimental Data Snapshot

Method: ELECTRON MICROSCOPY
Resolution: 2.63 Å
Aggregation State: PARTICLE
Reconstruction Method: SINGLE PARTICLE

wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

Calcium stabilization of a flexible N-terminal domain in a pentameric ligand-gated ion channel

Fan, C., Howard, R.J., Lindahl, E.

To be published.

Macromolecule Content

Total Structure Weight: 358.67 kDa
Atom Count: 23,492
Modelled Residue Count: 3,005
Deposited Residue Count: 3,210
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Neur_chan_LBD domain-containing protein	A, B, C, D, E	642	Desulfofustis sp. PB-SRB1	Mutation(s): 0 Gene Names: N839_03575
UniProt
Find proteins for V4JF97 (Desulfofustis sp. PB-SRB1) Explore V4JF97 Go to UniProtKB: V4JF97
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	V4JF97
Sequence Annotations Expand
Reference Sequence

Experimental Data & Validation

Experimental Data

Method: ELECTRON MICROSCOPY
Resolution: 2.63 Å
Aggregation State: PARTICLE
Reconstruction Method: SINGLE PARTICLE

EM Software:

Task	Software Package	Version
RECONSTRUCTION	cryoSPARC

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2025-04-09

Deposition Author(s):

Funding Organization	Location	Grant Number
Swedish Research Council		--

Revision History (Full details and data files)

Version 1.0: 2025-04-09
Type: Initial release

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.